mouse anti cyp19a1  (Novus Biologicals)

Journal: BMC genomics

Article Title: Aromatase reduces sperm motility by down-regulating the expression of proteins related to ATP synthesis in seminal plasma extracellular vesicles.

doi: 10.1186/s12864-025-11500-5

Figure Lengend Snippet: Fig. 1 Cyp19a1 expression level detection and semen quality assessment of control group and LV-CYP19A1 group roosters. A Construction of Cyp19a1 over-expression vector. B Diagram of injection of lentivirus carrying Cyp19a1 over-expression vector into rooster testis. C qRT-PCR results of Cyp19a1 in control group and LV-CYP19A1 group. D WB results of Cyp19a1 in control group and LV-CYP19A1 group..a−b Different letters within the same row show significant differences among the groups (P < 0.05)

Article Snippet: The PVDF membranes were incubated with primary antibodies CYP19A1 (BIO-RAD, MCA2077S), CD9 (abclonal, A19027), TSG101 (abcam, ab133586), and ALIX (abclonal, A2215) proteins at 4°C overnight.

Techniques: Expressing, Control, Over Expression, Plasmid Preparation, Injection, Quantitative RT-PCR

Journal: BMC genomics

Article Title: Aromatase reduces sperm motility by down-regulating the expression of proteins related to ATP synthesis in seminal plasma extracellular vesicles.

doi: 10.1186/s12864-025-11500-5

Figure Lengend Snippet: Fig. 2 Serum and seminal plasma hormone levels detection of control group and LV-CYP19A1 group roosters. Seminal plasma T (A), E2 (C), and T/E2 (E) levels of control group and LV-CYP19A1 group. Serum T (B), E2 (D), and T/E2 (F) levels of control group and LV-CYP19A1 group. a−b Different letters within the same row show significant differences among the groups

Article Snippet: The PVDF membranes were incubated with primary antibodies CYP19A1 (BIO-RAD, MCA2077S), CD9 (abclonal, A19027), TSG101 (abcam, ab133586), and ALIX (abclonal, A2215) proteins at 4°C overnight.

Techniques: Clinical Proteomics, Control

Journal: BMC genomics

Article Title: Aromatase reduces sperm motility by down-regulating the expression of proteins related to ATP synthesis in seminal plasma extracellular vesicles.

doi: 10.1186/s12864-025-11500-5

Figure Lengend Snippet: Fig. 3 Morphology, particle size, and marker proteins identification of seminal plasma extracellular vesicles (SPEV). A-B Morphological characteristics of SPEV in control group and LV-CYP19A1 group. C-D Particle size distribution of SPEV in control group and LV-CYP19A1 group. E Western Blot (WB) analysis of the common SPEV marker proteins

Article Snippet: The PVDF membranes were incubated with primary antibodies CYP19A1 (BIO-RAD, MCA2077S), CD9 (abclonal, A19027), TSG101 (abcam, ab133586), and ALIX (abclonal, A2215) proteins at 4°C overnight.

Techniques: Marker, Clinical Proteomics, Control, Western Blot

Journal: BMC genomics

Article Title: Aromatase reduces sperm motility by down-regulating the expression of proteins related to ATP synthesis in seminal plasma extracellular vesicles.

doi: 10.1186/s12864-025-11500-5

Figure Lengend Snippet: Fig. 5 GO and KEGG analysis of differentially expressed proteins (DEPs) between control group and LV-CYP19A1 group. A GO analysis of DEPs between control group and LV-CYP19A1 group. B KEGG analysis of DEPs between control group and LV-CYP19A1 group

Article Snippet: The PVDF membranes were incubated with primary antibodies CYP19A1 (BIO-RAD, MCA2077S), CD9 (abclonal, A19027), TSG101 (abcam, ab133586), and ALIX (abclonal, A2215) proteins at 4°C overnight.

Techniques: Control

Journal: BMC genomics

Article Title: Aromatase reduces sperm motility by down-regulating the expression of proteins related to ATP synthesis in seminal plasma extracellular vesicles.

doi: 10.1186/s12864-025-11500-5

Figure Lengend Snippet: Fig. 4 Identification of differentially expressed proteins (DEPs) between control group and LV-CYP19A1 group. A Principal component analysis (PCA) of DEPs between control group and LV-CYP19A1 group. B Volcanic maps of DEPs between control group and LV-CYP19A1 group. C Heatmap of DEPs between control group and LV-CYP19A1 group

Article Snippet: The PVDF membranes were incubated with primary antibodies CYP19A1 (BIO-RAD, MCA2077S), CD9 (abclonal, A19027), TSG101 (abcam, ab133586), and ALIX (abclonal, A2215) proteins at 4°C overnight.

Techniques: Control

Journal: BMC genomics

Article Title: Aromatase reduces sperm motility by down-regulating the expression of proteins related to ATP synthesis in seminal plasma extracellular vesicles.

doi: 10.1186/s12864-025-11500-5

Figure Lengend Snippet: Fig. 6 Key protein-biological process, protein-pathway, and protein–protein interaction (PPI) analysis of differentially expressed proteins (DEPs). A Key protein-biological process analysis of DEPs between control group and LV-CYP19A1 group. B PPI analysis of DEPs involved in key biological processes between control group and LV-CYP19A1 group. C Key protein-pathway analysis of DEPs between control group and LV-CYP19A1 group. D PPI analysis of DEPs involved in key pathways between control group and LV-CYP19A1 group. Orange diamonds represent the key pathways and biological processes; Green triangles represent the key proteins; Orange inverted triangles represent proteins that interact with individual key proteins; Pink octagon represent proteins that interact with multiple key proteins

Article Snippet: The PVDF membranes were incubated with primary antibodies CYP19A1 (BIO-RAD, MCA2077S), CD9 (abclonal, A19027), TSG101 (abcam, ab133586), and ALIX (abclonal, A2215) proteins at 4°C overnight.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Charged Amino Acids in the Transmembrane Helix Strongly Affect the Enzyme Activity of Aromatase

doi: 10.3390/ijms25031440

Figure Lengend Snippet: Measurement of enzymatic activity of recombinant CYP19A1 wild-type and mutant proteins. In two sets of experiments, 3 H-testosterone and 3 H-androstenedione were converted to 3 H-estradiol and 3 H-estrone, respectively, under standardized reaction conditions. After a reaction time of 30 min, the products were separated via HPLC and the 3 H activity was measured in the collected fractions. The X -axis shows the mean values of the 3 H activity in the androgen substrates and in the estrogen products of three experiments, each as a percentage of the input 3 H activity. Error bars indicate standard errors of the mean.

Article Snippet: The anti-CYP19A1 mouse monoclonal antibody raised against polypeptide AA376-390 of human CYP19A1 (clone H4, SM2222P, Acris Antibodies, Herford, Germany) and the anti-ACTB mouse monoclonal antibody against chicken beta-actin (sc-47778, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) served as the primary antibodies.

Techniques: Activity Assay, Recombinant, Mutagenesis

Journal: eLife

Article Title: Steroidogenesis and androgen/estrogen signaling pathways are altered in in vitro matured testicular tissues of prepubertal mice

doi: 10.7554/elife.85562

Figure Lengend Snippet: Figure 5. The expression of aromatase and estrogen signaling is impaired after 30 days of organotypic culture. (A) Relative mRNA levels of Cyp19a1 (normalized to Gapdh and Actb) and (B) relative protein levels of CYP19A1 (normalized to ACTB) during mouse postnatal development (6 dpp, 22 dpp, and 36 dpp) and in in vitro cultured fresh testicular tissues (FT) or controlled slow freezing (CSF) tissues (D16 and D30). The bands on the western blot correspond to different isoforms of CYP19. (C) Representative images of CYP19A1 expression at 6 dpp, 22 dpp, and 36 dpp and at corresponding in vitro time points (D16 and D30). A representative image of a negative control, carried out by omitting the primary antibody, is also shown. Testicular tissue sections were counterstained with Hematoxylin. Scale: 15 µm. Arrow: Leydig cells. Arrowheads: elongated spermatids. (D) Aromatase activity (normalized to tissue weight). (E–G) Relative mRNA levels of Esr1, Esr2, and Gper1 (normalized to Gapdh and Actb). (H) Relative mRNA levels of Faah (normalized to Gapdh and Actb) and (I) relative protein levels of fatty acid amide hydrolase (FAAH) (normalized to ACTB). The second band at 80 kDa is an isoform of FAAH (Q8BRM1, UniProtKB). Data are presented as means ± SEM with n=4 biological replicates for each group. A value of *p<0.05 was considered statistically significant.

Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Biological sample (Mus musculus, male) Testis CD- 1 Mice from Charles River Freshly isolated from male Mus musculus (CD- 1) Antibody Anti- 3β-HSD (mouse monoclonal) Santa Cruz Biot. sc- 515120 (AF488) IF (1:100), WB (1:1000) Antibody Anti-β-Actin (mouse monoclonal) Abcam ab8226 WB (1:5000) Antibody Anti- AR (rabbit monoclonal) Abcam ab133273 IF (1:100), WB (1:5000) Antibody Anti- CC3 (rabbit polyclonal) Abcam ab49822 IF (1:200) Antibody Anti- CYP17A1 (rabbit polyclonal) Abcam ab231794 IF (1:200), WB (1:10000) Antibody Anti- CYP19A1 (mouse monoclonal) BioRad MCA2077S IHC (1:50), WB (1:250) Antibody Anti- FAAH (rabbit polyclonal) Proteintech 17909–1- AP WB (1:1000) Antibody Anti- Ki67 (rabbit monoclonal) Abcam ab16667 IF (1:100) Antibody Anti- mouse Alexa 488 (goat) Abcam ab150113 IF (1:200) Antibody Anti- rabbit Alexa 488 (goat) Abcam ab150077 IF (1:200) Antibody Anti- rabbit Alexa 594 (goat) Abcam ab150080 IF (1:200) Antibody Anti- mouse HRP (goat) Invitrogen 31430 WB (1:5000) Antibody Anti- rabbit HRP (goat) Invitrogen A16110 WB (1:5000) Commercial assay or kit RNeasy Micro kit Qiagen 74004 Commercial assay or kit qScript cDNA SuperMix QuantaBio 95048 Commercial assay or kit Lipid Extraction kit Abcam ab211044 Commercial assay or kit Cholesterol/Cholesteryl Ester assay kit Abcam ab65359 Commercial assay or kit Progesterone ELISA kit Cayman Chemical Company 582601 Commercial assay or kit Estradiol ELISA kit Cayman Chemical Company 501890 Commercial assay or kit Aromatase (CYP19A) Activity assay kit Abcam ab273306 Moutard et al. eLife 2023;12:RP85562.

Techniques: Expressing, In Vitro, Cell Culture, Western Blot, Negative Control, Activity Assay